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ne52 qq57  (MedChemExpress)


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    Structured Review

    MedChemExpress ne52 qq57
    Ne52 Qq57, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ne52+qq57/NE+52-QQ57/pm40211064-204-3-4
    Average 93 stars, based on 7 article reviews
    ne52 qq57 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Structural basis of stepwise proton sensing-mediated GPCR activation.
    Article Snippet: For purification, cells were lysed and washed with low-salt buffer containing 10mM HEPES, pH 7.5, 20 mM KCl, 10 mM MgCl2 and EDTAfree protease inhibitor cocktail (Roche), followed by washing with high-salt buffer containing 10mM HEPES, pH 7.5, 20 mM KCl, 1 M NaCl, 10 mM MgCl2 and EDTA-free protease inhibitor cocktail. .. Then, 100 μM NE52-QQ57 (MCE, HY-101784) was added and incubated with purified membranes at 4 °C for 2 h, followed by addition of 2 mg/mL iodoacetamide for another 1 h. GPR4 protein was extracted with 1% (w/v) LMNG, 0.2% (w/v) CHS for 2.5 h at 4 °C. ..

    Article Title: Structural basis of stepwise proton sensing-mediated GPCR activation
    Article Snippet: For purification, cells were lysed and washed with low-salt buffer containing 10 mM HEPES, pH 7.5, 20 mM KCl, 10 mM MgCl 2 and EDTA-free protease inhibitor cocktail (Roche), followed by washing with high-salt buffer containing 10 mM HEPES, pH 7.5, 20 mM KCl, 1 M NaCl, 10 mM MgCl 2 and EDTA-free protease inhibitor cocktail. .. Then, 100 μM NE52-QQ57 (MCE, HY-101784) was added and incubated with purified membranes at 4 °C for 2 h, followed by addition of 2 mg/mL iodoacetamide for another 1 h. GPR4 protein was extracted with 1% (w/v) LMNG, 0.2% (w/v) CHS for 2.5 h at 4 °C. ..

    Purification:

    Article Title: Structural basis of stepwise proton sensing-mediated GPCR activation.
    Article Snippet: For purification, cells were lysed and washed with low-salt buffer containing 10mM HEPES, pH 7.5, 20 mM KCl, 10 mM MgCl2 and EDTAfree protease inhibitor cocktail (Roche), followed by washing with high-salt buffer containing 10mM HEPES, pH 7.5, 20 mM KCl, 1 M NaCl, 10 mM MgCl2 and EDTA-free protease inhibitor cocktail. .. Then, 100 μM NE52-QQ57 (MCE, HY-101784) was added and incubated with purified membranes at 4 °C for 2 h, followed by addition of 2 mg/mL iodoacetamide for another 1 h. GPR4 protein was extracted with 1% (w/v) LMNG, 0.2% (w/v) CHS for 2.5 h at 4 °C. ..

    Article Title: Structural basis of stepwise proton sensing-mediated GPCR activation
    Article Snippet: For purification, cells were lysed and washed with low-salt buffer containing 10 mM HEPES, pH 7.5, 20 mM KCl, 10 mM MgCl 2 and EDTA-free protease inhibitor cocktail (Roche), followed by washing with high-salt buffer containing 10 mM HEPES, pH 7.5, 20 mM KCl, 1 M NaCl, 10 mM MgCl 2 and EDTA-free protease inhibitor cocktail. .. Then, 100 μM NE52-QQ57 (MCE, HY-101784) was added and incubated with purified membranes at 4 °C for 2 h, followed by addition of 2 mg/mL iodoacetamide for another 1 h. GPR4 protein was extracted with 1% (w/v) LMNG, 0.2% (w/v) CHS for 2.5 h at 4 °C. ..

    Cell Culture:

    Article Title: The proton-activated G protein-coupled receptor GPR4 regulates the development of osteoarthritis via modulating CXCL12/CXCR7 signaling.
    Article Snippet: .. They were then cultured in DMEM containing 10% FBS, supplemented with NE52-QQ57 (MedChemExpress, HY-1D1784, 10 μM) or vehicle for 1 week. .. Cartilage explants were then collected for histological analysis and mRNA expression level examination, while supernatant was collected for testing using the 1, 9, dimethyl methylene blue (DMMB) (Sigma-Aldrich, 341088-1G) assay.

    Article Title: The proton-activated G protein-coupled receptor GPR4 regulates the development of osteoarthritis via modulating CXCL12/CXCR7 signaling
    Article Snippet: .. They were then cultured in DMEM containing 10% FBS, supplemented with NE52-QQ57 (MedChemExpress, HY-1D1784, 10 μM) or vehicle for 1 week. .. Cartilage explants were then collected for histological analysis and mRNA expression level examination, while supernatant was collected for testing using the 1, 9, dimethyl methylene blue (DMMB) (Sigma-Aldrich, 341088-1G) assay.



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